Couchman, L. published the artcileAn automated method for the measurement of a range of tyrosine kinase inhibitors in human plasma or serum using turbulent flow liquid chromatography-tandem mass spectrometry, Synthetic Route of 863127-77-9, the publication is Analytical and Bioanalytical Chemistry (2012), 403(6), 1685-1695, database is CAplus and MEDLINE.
Tyrosine kinase inhibitors (TKIs) are used to treat a number of cancers, including chronic myeloid leukemia and hepatocellular carcinoma. Therapeutic drug monitoring (TDM) may be indicated to (1) monitor adherence, (2) guide dosage, and (3) minimise the risk of drug-drug interactions and dose-related toxicity. Online, automated sample preparation provided by TurboFlow technol. (ThermoFisher Scientific) in conjunction with the sensitivity and selectivity of tandem mass spectrometry (MS/MS) detection may be applied to the anal. of single drugs and metabolites. We report the use of TurboFlow LC-MS/MS for the anal. of nine TKIs and metabolites (imatinib, N-desmethylimatinib, dasatinib, nilotinib, erlotinib, gefitinib, lapatinib, sorafenib, sunitinib) in human plasma or serum for TDM purposes. An Aria Transcend TLX-II system coupled with a TSQ Vantage was used. Samples (50 μL) were vortex mixed with internal standard solution (150 μL imatinib-D8, gefitinib-D8, sunitinib-D10, and nilotinib-13C215N2 in acetonitrile) and, after centrifugation 100 μL supernatant were injected directly onto a 50 × 0.5-mm Cyclone TurboFlow column. Analytes were focussed onto a 50 × 2.1-mm (3 μm) Hypersil GOLD anal. column and eluted with an acetonitrile/water gradient. Analytes were monitored in selected reaction monitoring mode (pos. APCI). Total anal. time was 7 min without multiplexing. Calibration was linear (R2 > 0.99) for all analytes. Inter- and intra-assay precision (in percent relative standard deviation, RSD) was <11 % and accuracy 89-117 % for all analytes. No matrix effects were observed This method is suitable for high-throughput TDM in patients undergoing chronic therapy with TKIs and has been utilized in the anal. of clin. samples.
Analytical and Bioanalytical Chemistry published new progress about 863127-77-9. 863127-77-9 belongs to piperazines, auxiliary class TGF-beta/Smad,Bcr-Abl,Natural product, name is N-(2-Chloro-6-methylphenyl)-2-((6-(4-(2-hydroxyethyl)piperazin-1-yl)-2-methylpyrimidin-4-yl)amino)thiazole-5-carboxamide hydrate, and the molecular formula is C22H28ClN7O3S, Synthetic Route of 863127-77-9.
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https://en.wikipedia.org/wiki/Piperazine,
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