A (GTG)5 PCR fingerprinting of multidrug resistant Escherichia coli bacteria isolates from hospital in Ouagadougou, Burkina Faso was written by Kabore, Boukare;Ouedraogo, Ganame Abasse;Cisse, Hama;Ouedraogo, Henri S.;Sampo, Emmanuel;Zongo, Koudbi Jacob;Zeba, Boukare;Traore, Yves;Gnankine, Olivier;Sanou, Idrissa;Savadogo, Aly. And the article was included in BMC Microbiology in 2022.Related Products of 70458-96-7 The following contents are mentioned in the article:
Escherichia coli (E. coli) is the most common bacterial species implicated in various types of infections including septicemia, gastroenteritis, urinary tract infections, meningitis and others pathologies. These involve several bacterial clones with multidrug resistance making them difficult to treat. The aims of this study was to perform mol. typing of E. coli strains using universal primer (GTG)5. In this study, 53 E. coli strains were collected from inpatients and outpatients. The test of antimicrobial sensibility was realized using CA-SFM /EUCAST method and strains were identified by conventional microbiol. tests. The carbapenemase-producing strains were demonstrated by phenotypic method. Bacterial DNA was extracted by boiling method. (GTG)5-PCR was used for strain subtyping. The DendroUPGMA software was used for grouping of strains from the genetic fingerprints obtained by (GTG)5-PCR. Antibiotic susceptibility test revealed that all strains were multi-drug resistant (MDR). Its strains showed resistance to at least three different families of antibiotics. Of this MDR strains, only one was a metallo-β-lactamase producer. The dendrogram obtained using genetic fingerprinting allowed the E. coli strains to be grouped into 22 clusters (G1 to G22). The (GTG)5-PCR assay enabled rapid mol. typing of E. coli strains. The strains of E. coli typed in this study would belong to different clones. This study involved multiple reactions and reactants, such as 1-Ethyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid (cas: 70458-96-7Related Products of 70458-96-7).
1-Ethyl-6-fluoro-4-oxo-7-(piperazin-1-yl)-1,4-dihydroquinoline-3-carboxylic acid (cas: 70458-96-7) belongs to piperazine derivatives. The piperazine scaffold is often found in biologically active compounds in different therapeutic areas. These therapeutic areas include antifungals, antidepressants, antivirals, and serotonin receptor (5-HT) antagonists/agonists. Outside the body, piperazine has a remarkable power to dissolve uric acid and producing a soluble urate, but in clinical experience it has not proved equally successful. Related Products of 70458-96-7
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics