With the rapid development and complex challenges of chemical substances, new drug synthesis pathways are usually the most effective.7365-45-9,2-(4-(2-Hydroxyethyl)piperazin-1-yl)ethanesulfonic acid,as a common compound, the synthetic route is as follows.
7365-45-9, General procedure: Larger Scale Production of Nucleotide Analogs. (0230) The process described for small-scale batches is scalable to larger batches by using proportionate amounts of compounds. As an alternate to the small-scale batches described above, larger scale batches were prepared. Here, reaction mixtures having a total volume of 0.25 ml (>10× the volume of the small-scale batch) and containing 40 mM of a reagent from Table 1, 10 mM magnesium acetate, 80 muM dATP, 50 mug/ml BSA, and 5.84 muM p41 subunit (containing 0.64 M glycerol following dilution in the glycerol diluent described above) were incubated for 30 min at 70 C. Incubation with the p41 subunit and TLC analysis on PEI plates showed greater than 60% conversion of starting material (i.e., the reagent and dNTP) into nucleotide analog products (dNMP derivatives). Experiments were also performed using heavy isotopes incorporated in dNTP, but the presence of a heavy isotope did not alter the utilization of dNTP in the reaction. (0231) Samples from reaction mixtures were analyzed using a capillary reverse phase liquid chromatography system coupled to an Orbitrap Discovery mass spectrometer in negative mode. Negative mode nanospray mode was set to -1.5 kV, and chromatographic flow rate was <20 nl/min. Fragmentation was achieved using high collision dissociation at 45% energy. Isotope-labeled samples were run both individually and combined. By analyzing the mass difference between heavy and light fragmentation pattern, the identity of each fragmentation peak was established.
As the paragraph descriping shows that 7365-45-9 is playing an increasingly important role.
Reference：
Patent; THE UNITED STATES OF AMERICA, AS REPRESENTED BY THE SECRETARY OF COMMERCE; MEMORIAL SLOAN KETTERING CANCER CENTER; UNIVERSITY OF MARYLAND; Marino, John P.; Kelman, Zvi; Hurwitz, Jerard; Giulian, Gary G.; (45 pag.)US9534243; (2017); B2;,
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