Confocal Fluorescence Detection Expanded to UV Excitation: The First Continuous Fluorimetric Assay of Human Steroid Sulfatase in Nanoliter Volume was written by Billich, Andreas;Bilban, Melitta;Meisner, Nicole-Claudia;Nussbaumer, Peter;Neubauer, Andreas;Jaeger, Stefan;Auer, Manfred. And the article was included in Assay and Drug Development Technologies in 2004.Reference of 162046-66-4 This article mentions the following:
Steroid sulfatase is an enzyme that currently enjoys considerable interest as a potential drug target in the treatment of estrogen- and androgen-dependent diseases, in particular breast cancer. We have purified human steroid sulfatase to apparent homogeneity from recombinant Chinese hamster ovary cells, and we established an assay with a new fluorogenic substrate, 3,4-benzocoumarin-7-O-sulfate (1). Substrate 1 features a Km value of 22.5 μM, which is close to the value for the natural substrate dehydroepiandrosterone sulfate (26 μM) and much lower than the Km values of other synthetic substrates (276-736 μM). Importantly, the cleavage of substrate 1 can be monitored continuously during the enzymic cleavage, since a change in fluorescence intensity is detectable at the pH where the enzyme is active; in contrast, all other synthetic substrates described so far require alkalization to reveal a measurable absorbance or fluorescence signal. The adaptation of the assay to the 96-well format allows continuous monitoring of multiple wells in a microplate fluorescence reader. Applications of the assay for the determination of IC50 and Ki values of novel steroid sulfatase inhibitors are presented. Most importantly the assay was transferred to the nanoscale format (1-μl assay volume) in 2080-well plates with confocal fluorescence detection. This miniaturization will permit screening with a min. throughput of 20,000 compounds per day. The system presented demonstrates that the confocal detection platform used for nanoscreening can be successfully adapted to assays for which conventional UV dyes like coumarins are necessary. This strongly broadens the application range of confocal readers in drug screening. In the experiment, the researchers used many compounds, for example, 4-(4-(tert-Butoxycarbonyl)piperazin-1-yl)benzoic acid (cas: 162046-66-4Reference of 162046-66-4).
4-(4-(tert-Butoxycarbonyl)piperazin-1-yl)benzoic acid (cas: 162046-66-4) belongs to piperazine derivatives. Simple N-substituted piperazines have been found in many drug molecules. Two common salts in the form of which piperazine is usually prepared for pharmaceutical or veterinary purposes are the citrate, 3C4H10N2.2C6H8O7 (i.e. containing 3 molecules of piperazine to 2 molecules of citric acid), and the adipate, C4H10N2.C6H10O4 (containing 1 molecule each of piperazine and adipic acid).Reference of 162046-66-4
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics