The author of 《Development of a multitasking fluorescent probe for differentiating G-Quadruplex structures》 were Hu, Ming-Hao; Chen, Xiao; Tan, Jia-Heng. And the article was published in Dyes and Pigments in 2019. HPLC of Formula: 109-01-3 The author mentioned the following in the article:
G-quadruplexes exhibit extensive structural polymorphism, which are highly related to their biol. functions. To date, multitasking probes that can simultaneously discriminate among parallel, antiparallel G-quadruplexes and single-/double-stranded nucleic acids have never been reported. The authors designed a probe IZNP-2 (I) based on the photoinduced electron transfer (PeT) mechanism. Conformation anal. firstly revealed that IZNP-2 was a smart probe, of which the fluorescence varied according to its mol. conformations. Then, fluorescence assays demonstrated that IZNP-2 could not only differentiate between parallel and antiparallel G-quadruplexes, but also discriminate antiparallel G-quadruplexes from single-/double-stranded nucleic acids. To understand this multitasking ability, the authors performed various experiments, including absorption titrations, lifetime experiments, Job plot assays and 2-Ap experiments, to study the binding modes, which suggested that IZNP-2 might exhibit “”Stretched””, “”Semi-stretched”” and “”Stacked”” conformations when targeting different nucleic acid topologies, alleviating the PeT to different extents and thus inducing differentiable fluorescence. Furthermore, the authors broadened the application of IZNP-2 in discriminating between multimeric and monomeric G-quadruplexes. To the best of the authors’ knowledge, such a study provides a first example of developing a multitasking fluorescent probe for differentiating different G-quadruplex structures by exploiting the PeT mechanism. In the experiment, the researchers used 1-Methylpiperazine(cas: 109-01-3HPLC of Formula: 109-01-3)
1-Methylpiperazine(cas: 109-01-3) can be used as mimic template in the preparation of molecularly imprinted microspheres (MIMs). It was also used to prepare the difunctional strong anion-exchange stationary phase from a 1,4-diazacyclohexane derivative..HPLC of Formula: 109-01-3
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics