《Synthesis, characterization and carbonic anhydrase I and II inhibitory evaluation of new sulfonamide derivatives bearing dithiocarbamate》 was published in European Journal of Medicinal Chemistry in 2020. These research results belong to Saglik, Begum Nurpelin; Osmaniye, Derya; Cevik, Ulviye Acar; Levent, Serkan; Cavusoglu, Betul Kaya; Buyukemir, Oya; Nezir, Deniz; Karaduman, Abdullah Burak; Ozkay, Yusuf; Koparal, Ali Savas; Beydemir, Sukru; Kaplancikli, Zafer Asim. Formula: C5H12N2 The article mentions the following:
In this study, novel dithiocarbamate-sulfonamide derivatives I [R = Me, cyclohexyl, 4-O2NC6H4, etc.] were synthesized to investigate their inhibitory activity on purified human carbonic anhydrase (hCA) I and II. The IC50 and Ki values of the compounds were calculated to compare their inhibition profiles on hCA I and II isoenzymes. Acetazolamide was used as the standard inhibitor in the enzyme inhibition assay. Compounds I [R = Me, 4-MeC6H4CH2, CH2CH2NMe2, CH2CH2CH2NMe2, 2-pyridinyl, 2-pyrimidinyl] showed notable inhibitory effects against hCA I and II. Among these compounds, compound I [R = CH2CH2CH2NMe2] was found to be the most active derivate against both the hCA I and II enzymes with Ki values of 0.032 ± 0.001μM and 0.013 ± 0.0005μM, resp. The cytotoxicity of compounds I [R = Me, 4-MeC6H4CH2, CH2CH2NMe2, CH2CH2CH2NMe2, 2-pyridinyl, 2-pyrimidinyl] toward NIH/3T3 (mouse embryonic fibroblast cell line) was observed and the compounds were found to be non-cytotoxic. Furthermore, mol. docking studies were performed to investigate the interaction types between compound I [R = CH2CH2CH2NMe2] and the hCA I and II enzymes. As a result of this study a novel and potent class of CA inhibitors with good activity potential were identified. The experimental part of the paper was very detailed, including the reaction process of 1-Methylpiperazine(cas: 109-01-3Formula: C5H12N2)
1-Methylpiperazine(cas: 109-01-3) can be used as mimic template in the preparation of molecularly imprinted microspheres (MIMs). It was also used to prepare the difunctional strong anion-exchange stationary phase from a 1,4-diazacyclohexane derivative..Formula: C5H12N2
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics