Piperazine is an organic compound that consists of a six-membered ring containing two nitrogen atoms at opposite positions in the ring. 103-76-4, formula is C6H14N2O, Name is N-(2-Hydroxyethyl)piperazine. Piperazine exists as small alkaline deliquescent crystals with a saline taste. HPLC of Formula: 103-76-4.
Xiao, Shengnan;Wang, Xude;Xu, Lei;Miao, Dongyu;Li, Tao;Su, Guangyue;Zhao, Yuqing research published 《 Novel ginsenoside derivatives have shown their effects on PC-3 cells by inducing G1-phase arrest and reactive oxygen species-mediate cell apoptosis》, the research content is summarized as follows. In this study, piperazine groups were introduced into ginsenoside to enhance its ability to induce Reactive Oxygen Species (ROS) production and apoptosis in cancer cells. In total, 27 ginsenoside piperazine derivatives, e.g. 20(R)-pananxadiol-12β-hydroxy-3β-yl-[4-pyridin-4-yl-piperazin-1-yl]-acetate I, were synthesized and tested for their anti-proliferative activity in cancer cell lines by MTT assay. The results showed that compounds, e.g. I, had significant inhibitory effects on cancer cell growth. Compound I showed the strongest anti-proliferative effect on PC-3 cells with an IC50 of 1.98 ± 0.34μM. Compound I could also induce G1-phase arrest and apoptosis in PC-3 cells, with apoptosis rates of 8.1%, 41% and 56.1% observed at 5, 10 and 20μM, resp. Compound I also significantly enhanced the intracellular fluorescence of ROS sensitive substrates, with a fluorescence intensity ratio of 23.1% observed in treated cells, indicative of ROS production Following N-acetylcysteine treatment, apoptotic rates of the cancer cell lines decreased from 38.9% to 7.3%, and the expression of Cl-PARP, Cl-Caspase-3 and Cl-Caspase-9 also decreased, confirming that compound I induced apoptosis through ROS induction. Compound I also stimulated the translocation of Bax from the cytoplasm to the mitochondria, which enhanced Cytochrome C (Cyt C) release, and increased the expression of the apoptotic markers Cl-PARP, Cl-Caspase-3, and Cl-Caspase-9 in PC-3 cells. Taken together, these data reveal the anti-cancer effects of compound I that enhance ROS production, and then induce apoptosis through mitochondrial pathway.
103-76-4, 1-(2-Hydroxyethyl)piperazine is a useful research compound. Its molecular formula is C6H14N2O and its molecular weight is 130.19 g/mol. The purity is usually 95%.
1-(2-Hydroxyethyl)piperazine is a chemical solvent that is used for the absorption of amines and other compounds. It has been shown to have a high affinity for acidic substances, which may be due to its ability to form hydrogen bonds with them. 1-(2-Hydroxyethyl)piperazine is also used in sample preparation for the analysis of amines and other compounds in fruit extracts. The solubility of 1-(2-Hydroxyethyl)piperazine depends on the experimental conditions, including pH, temperature, and pressure. FT-IR spectroscopy has been used to measure the vibrational modes of 1-(2-Hydroxyethyl)piperazine molecules. The IR spectrum revealed that this compound contains a hydroxyl group and two fatty acid chains with one or more hydroxyl groups at their terminal end. The nmr spectrum showed that 1-(2-Hydroxyethyl)piperazine contains an NH proton as well as, HPLC of Formula: 103-76-4
Referemce:
Piperazine – Wikipedia,
Piperazines – an overview | ScienceDirect Topics