With the rapid development and complex challenges of chemical substances, new drug synthesis pathways are usually the most effective.103-76-4,N-(2-Hydroxyethyl)piperazine,as a common compound, the synthetic route is as follows.
1 mol of N-hydroxyethylpiperazine and25% by mass aqueous solution of ammonium vinyl sulfonate (containing 1.10 mol of ammonium vinyl sulfonate)Was added to a 1000 mL four-necked flask equipped with a reflux tube,The addition reaction is carried out with sufficient stirring;The reaction was carried out at 60 C for 1.5 hours,And then gradually warming boiling reflux,And continue to react for 2.5 hours;then,The reaction was quenched to give a reaction mother liquor containing 4-hydroxyethylpiperazine ethanesulfonate.By high performance liquid chromatography,The yield of ammonium 4-hydroxyethylpiperazine ethanesulfonate was calculated to be 89.4%.A reaction mother liquor containing 0.5 mol HEPES-NH4 was placed in a 500 mL beaker,Under stirring,27.5 g of concentrated sulfuric acid (98 wt%) was slowly added dropwise at room temperature,Then stir,And acidified at 0 C for 1 hour.Into the low temperature thermostat bath, cooling to -15 ,Cooling crystallization for 0.5 hours,Remove the sodium sulfate by filtration.The filtrate was placed in a beaker,Constantly stirring,Slowly add 3.0 g of Ba (OH) 2,Reaction for 1 hour,Remove the remaining sulfate.Adding 5 g of activated carbon decolorization and filtering by filtration to obtain the filtrate,By rotary evaporation to anhydrous,To obtain a solid primary purified product.The solid was washed twice with 500 ml of methanol,500ml ethanol once,And then vacuum drying,Get high purity HEPES.
103-76-4, 103-76-4 N-(2-Hydroxyethyl)piperazine 7677, apiperazines compound, is more and more widely used in various fields.
Reference：
Patent; Shandong University of Technology; Cui Hongyou; Wang Jiangang; Zhu Liwei; Liu Ransheng; Yang Yong; Wang Yang; (8 pag.)CN104803949; (2017); B;,
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